Coding

Part:BBa_K1745002

Designed by: Benjamin Hsu   Group: iGEM15_UChicago   (2015-09-17)

AANAT Expression

Expression and possibly secretion of enzyme AANAT (aralkylamine N-acetyltransferase) [ Homo sapiens (human) ]. AANAT catalyzes Serotonin into N-acetylserotonin, a Melatonin precursor, and it is the rate-limiting step in Melatonin production. (Zheng 2002) Melatonin (N-acetyl-5-methoxytryptamine) is responsible for regulating circadian rhythms such as the sleep cycle and seasonal behaviors such as color change and reproduction in animals.(Lincoln 2003) In humans, AANAT is secreted in the Pineal gland. This construct is His-tagged.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 115
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 509
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None